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GrainGenes Marker Report: k00122

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Locus
k00122
Type
CAPS
Chromosome
6H
Map
Hv-HNxH602-6H204.7
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
baak14k12
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = AvaII
SNP = A/G
SNP structure (UNICODE) = GCATCCGCTGRGGCTGGTGCA
Parent 1 SNP sequence = GCATCCGCTGAGGCTGGTGCA
Parent 2 SNP sequence = GCATCCGCTGGGGCTGGTGCA
Recognized sequence for CAPs locus = GGWCC
SNP = T/C
SNP structure (UNICODE) = GCACCCTTGTYATCGTCCAAC
Parent 1 SNP sequence = GCACCCTTGTTATCGTCCAAC
Parent 2 SNP sequence = GCACCCTTGTCATCGTCCAAC



Probe for Locus
baak14k12
Locus
k00122
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = thylakoid-bound ascorbate peroxidase [Triticum aestivum]
Type
PCR
PCR primers
Left 5' - CAGAGCATTTTCATGGAGCA
Right 5' - CTATCCGAAGTTGGAGGACG
Sequence
BJ454752
PCR size
406