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GrainGenes Marker Report: k00256

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Locus	k00256
Type	CAPS
Chromosome	1H
Map	Hv-HNxH602-1H 80.6
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baak1j14
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = PstI SNP = G/A SNP structure (UNICODE) = ATCTTCTGCARGCACTTGTCG Parent 1 SNP sequence = ATCTTCTGCAGGCACTTGTCG Parent 2 SNP sequence = ATCTTCTGCAAGCACTTGTCG Recognized sequence for CAPs locus = CTGCAG
Probe for Locus	baak1j14
Locus	k00256
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = Glutamyl-tRNA reductase 2 (GluTR) pir T05734 glutamyl-tRNA reductase (EC 1.2.1.-) 2 - barley (fragment)
Type	PCR
PCR primers	Left 5' - GAAAAGCCTTCTCCATCTGC Right 5' - CCTTGGTCAGCAGCATTACA
Sequence	AV911817
PCR size	381