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GrainGenes Marker Report: k00527

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Locus	k00527
Type	CAPS
Chromosome	1H
Map	Hv-HNxH602-1H 127.9
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baak34k14
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = SalI SNP = G/A SNP structure (UNICODE) = CCGGCACGCCRTCGACCTTCA Parent 1 SNP sequence = CCGGCACGCCGTCGACCTTCA Parent 2 SNP sequence = CCGGCACGCCATCGACCTTCA Recognized sequence for CAPs locus = GTCGAC
Probe for Locus	baak34k14
Locus	k00527
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = expressed protein [Arabidopsis thaliana] pir C84809 hypothetical protein At2g38780 [imported] - Arabidopsis thaliana
Type	PCR
PCR primers	Left 5' - GAAGAGCAAGGAACACCTGC Right 5' - ATGAGTACCTCGATGGTGGC
Sequence	BJ485355
PCR size	395