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GrainGenes Marker Report: k00528

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Locus
k00528
Type
CAPS
Chromosome
6H
Map
Hv-HNxH602-6H204.7
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
baak34p14
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = BcnI
SNP = G/A
SNP structure (UNICODE) = CAATGTCCCGRAGGATACGGA
Parent 1 SNP sequence = CAATGTCCCGGAGGATACGGA
Parent 2 SNP sequence = CAATGTCCCGAAGGATACGGA
Recognized sequence for CAPs locus = CCSGG



Probe for Locus
baak34p14
Locus
k00528
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = putative translation initiation factor [Oryza sativa] gb AAK50586.1 putative translation initiation factor [Oryza sativa]
Type
PCR
PCR primers
Left 5' - TGTTTTCTCAGGGTGCACAG
Right 5' - CGAAGACTGGATCGAAGAGG
Sequence
BJ459169
PCR size
437