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GrainGenes Marker Report: k00614

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Locus	k00614
Type	CAPS
Chromosome	3H
Map	Hv-HNxH602-3H 36.1
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baak35n06
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 10 ] Restriction enzyme used for CAPS analysis = TspRI SNP = C/T SNP structure (UNICODE) = ACGGCACGGTYAGTGTCAGGT Parent 1 SNP sequence = ACGGCACGGTCAGTGTCAGGT Parent 2 SNP sequence = ACGGCACGGTTAGTGTCAGGT Recognized sequence for CAPs locus = CASTGNN SNP = T/G SNP structure (UNICODE) = ATTTTAGACAKTGTWTGGATC Parent 1 SNP sequence = ATTTTAGACATTGTTTGGATC Parent 2 SNP sequence = ATTTTAGACAGTGTATGGATC
Probe for Locus	baak35n06
Locus	k00614
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = expressed protein [Arabidopsis thaliana] gb AAL24160.1 At1g31300/T19E23_12 [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - AGTAGTGGGGAGTCGGGAAC Right 5' - GGCCAACTCAGAAGCTATGC
Sequence	BJ455230
PCR size	380