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GrainGenes Marker Report: k00623

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Locus
k00623
Type
CAPS
Chromosome
6H
Map
Hv-HNxH602-6H199.2
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
baak39o18
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = AfaI
SNP = A/G
SNP structure (UNICODE) = AGCCAACATGRTACTCTCTAT
Parent 1 SNP sequence = AGCCAACATGATACTCTCTAT
Parent 2 SNP sequence = AGCCAACATGGTACTCTCTAT
Recognized sequence for CAPs locus = GTAC



Probe for Locus
baak39o18
Locus
k00623
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = deoxymugineic acid synthase 1 [Hordeum vulgare subsp. vulgare]
Type
PCR
PCR primers
Left 5' - CCAAAAGGTAGCAAACAGGC
Right 5' - AGCAGTCGACGAGGACAAAT
Sequence
BJ460219
PCR size
391