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GrainGenes Marker Report: k00750

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Locus
k00750
Type
CAPS
Chromosome
4H
Map
Hv-HNxH602-4H250.7
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
baak41l23
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = HapII
SNP = T/A
SNP structure (UNICODE) = ACCTAACTAAWTCACACCAGC
Parent 1 SNP sequence = ACCTAACTAATTCACACCAGC
Parent 2 SNP sequence = ACCTAACTAAATCACACCAGC
Recognized sequence for CAPs locus = CCGG
SNP = G/A
SNP structure (UNICODE) = GTAATCAACTRATTCCTTTGG
Parent 1 SNP sequence = GTAATCAACTGATTCCTTTGG
Parent 2 SNP sequence = GTAATCAACTAATTCCTTTGG



Probe for Locus
baak41l23
Locus
k00750
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = putative Ca2+/H+-exchanging protein [Oryza sativa (japonica cultivar-group)]
Type
PCR
PCR primers
Left 5' - AACCTTGGTGTTCCATCAGC
Right 5' - TGGTGAAATTCAAAGGAGGG
Sequence
BJ546471
PCR size
478