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GrainGenes Marker Report: k00999

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Locus	k00999
Type	CAPS
Chromosome	4H
Map	Hv-HNxH602-4H 146.9
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baal17m22
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = MboI SNP = G/T SNP structure (UNICODE) = TCAGGTTGGAKCCATGGAGGT Parent 1 SNP sequence = TCAGGTTGGAGCCATGGAGGT Parent 2 SNP sequence = TCAGGTTGGATCCATGGAGGT Recognized sequence for CAPs locus = GATC
Probe for Locus	baal17m22
Locus	k00999
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = similar to polydom protein [Rattus norvegicus]
Type	PCR
PCR primers	Left 5' - TCAGTGTGTCTCCGCTTGTC Right 5' - GGAAATGGGTCGGTAGGAAT
Sequence	BJ473937
PCR size	360