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GrainGenes Marker Report: k01174

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Locus	k01174
Type	CAPS
Chromosome	2H
Map	Hv-HNxH602-2H 138.3
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baal33d23
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = SacII SNP = G/C SNP structure (UNICODE) = TGGTGGTGACSGCGGCCTGCA Parent 1 SNP sequence = TGGTGGTGACGGCGGCCTGCA Parent 2 SNP sequence = TGGTGGTGACCGCGGCCTGCA Recognized sequence for CAPs locus = CCGCGG
Probe for Locus	baal33d23
Locus	k01174
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = hypothetical protein~similar to Arabidopsis thaliana chromosome 3, AT3g01440 [Oryza sativa (japonica cultivar-group)]
Type	PCR
PCR primers	Left 5' - TGAGGGGAAAACAAAACACC Right 5' - CTTGGATGCTGATGCGTAGA
Sequence	BJ476267
PCR size	416