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GrainGenes Marker Report: k01927

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Locus
k01927
Type
CAPS
Chromosome
5H
Map
Hv-HNxH602-5H253.6
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bags10e02
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = BstUI
SNP = C/T
SNP structure (UNICODE) = CGTTGGTGCTYGCGTACGTCA
Parent 1 SNP sequence = CGTTGGTGCTCGCGTACGTCA
Parent 2 SNP sequence = CGTTGGTGCTTGCGTACGTCA
Recognized sequence for CAPs locus = CGCG



Probe for Locus
bags10e02
Locus
k01927
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = putative actin depolymerizing factor [Oryza sativa (japonica cultivar-group)]
Type
PCR
PCR primers
Left 5' - GGGATTTTCCAGCAACTGAA
Right 5' - GGTCCAAACTTGCATGGTCT
Sequence
BM370247
PCR size
367