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GrainGenes Marker Report: k02167

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Locus	k02167
Type	CAPS
Chromosome	1H
Map	Hv-HNxH602-1H 98
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bags15g01
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = BsmAI SNP = T/C SNP structure (UNICODE) = TGATCCGAGAYATGCAAAGAG Parent 1 SNP sequence = TGATCCGAGATATGCAAAGAG Parent 2 SNP sequence = TGATCCGAGACATGCAAAGAG Recognized sequence for CAPs locus = NNNNNGAGAC
Probe for Locus	bags15g01
Locus	k02167
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - ACTGACCACACCCATGGACT Right 5' - GCCATTTTCACCATACCACC
Sequence	AV920565
PCR size	463