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GrainGenes Marker Report: k02321

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Locus	k02321
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 201.4
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bags19i06
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = AfaI SNP = C/T SNP structure (UNICODE) = CCTAGTAGTAYGCAAAAACAC Parent 1 SNP sequence = CCTAGTAGTACGCAAAAACAC Parent 2 SNP sequence = CCTAGTAGTATGCAAAAACAC Recognized sequence for CAPs locus = GTAC
Probe for Locus	bags19i06
Locus	k02321
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = putative deoxyhypusine synthase [Oryza sativa (japonica cultivar-group)]
Type	PCR
PCR primers	Left 5' - CCAGATCAACAAACACCACG Right 5' - TGGAGTCATCTGAAGCGATG
Sequence	AV834538
PCR size	367