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GrainGenes Marker Report: k02582

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Locus	k02582
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 79.6
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bags21n10
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 10 ] Restriction enzyme used for CAPS analysis = HapII SNP = A/G SNP structure (UNICODE) = CCCATGACCGRTACGACTGGG Parent 1 SNP sequence = CCCATGACCGATACGACTGGG Parent 2 SNP sequence = CCCATGACCGGTACGACTGGG Recognized sequence for CAPs locus = CCGG SNP = C/N SNP structure (UNICODE) = TGGTGCTCGCC/NGGGCGGTAGT Parent 1 SNP sequence = TGGTGCTCGCCGGGCGGTAGT Parent 2 SNP sequence = TGGTGCTCGCNGGGCGGTAGT
Probe for Locus	bags21n10
Locus	k02582
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = putative proton pump interactor [Oryza sativa (japonica cultivar-group)]
Type	PCR
PCR primers	Left 5' - TCCAGAACCTGCACCTCTTT Right 5' - CGAGTGAAGTGCTACACCGA
Sequence	AV918674
PCR size	439