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GrainGenes Marker Report: k03033

Locus
k03033
Type
CAPS
Chromosome
2H
Map
Hv-HNxH602-2H118
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bags35c23
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = AluI
SNP = C/T
SNP structure (UNICODE) = CAACCATCAGYTGGCTACCAA
Parent 1 SNP sequence = CAACCATCAGCTGGCTACCAA
Parent 2 SNP sequence = CAACCATCAGTTGGCTACCAA
Recognized sequence for CAPs locus = AGCT



Probe for Locus
bags35c23
Locus
k03033
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = putative phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplast precursor [Oryza sativa (japonica cultivar-group)]
Type
PCR
PCR primers
Left 5' - CTCTCTCCAAAGCCGAAACA
Right 5' - GGGGCTAGATGCAGTCGTTA
Sequence
AV921451
PCR size
353