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GrainGenes Marker Report: k03227

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Locus	k03227
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 125.2
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bags3b05
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = HinfI SNP = C/T SNP structure (UNICODE) = AGAAAAGAGTYGACAGATTAT Parent 1 SNP sequence = AGAAAAGAGTCGACAGATTAT Parent 2 SNP sequence = AGAAAAGAGTTGACAGATTAT Recognized sequence for CAPs locus = GANTC
Probe for Locus	bags3b05
Locus	k03227
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = At2g23890 [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - TGATTTAGAGTCTCGCCGCT Right 5' - ATCCAGTTCCTCATCATCGC
Sequence	BJ459073
PCR size	355