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GrainGenes Marker Report: k03444

Locus
k03444
Type
CAPS
Chromosome
5H
Map
Hv-HNxH602-5H237.1
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bags9a01
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = AfaI
SNP = G/A
SNP structure (UNICODE) = CGAGCCCGGCRTACTGCACGA
Parent 1 SNP sequence = CGAGCCCGGCGTACTGCACGA
Parent 2 SNP sequence = CGAGCCCGGCATACTGCACGA
Recognized sequence for CAPs locus = GTAC



Probe for Locus
bags9a01
Locus
k03444
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = phosphatidic acid phosphatase family protein / PAP2 family protein [Arabidopsis thaliana]
Type
PCR
PCR primers
Left 5' - CGTGTATAGCAATAGGGAGCG
Right 5' - CATGATCCACCGGCATTAC
Sequence
AV920027
PCR size
356