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GrainGenes Marker Report: k03859

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Locus	k03859
Type	CAPS
Chromosome	2H
Map	Hv-HNxH602-2H 164.2
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bah27d18
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = AfaI SNP = T/- SNP structure (UNICODE) = TCAGTCTGAGT/-ACTAATCAAA Parent 1 SNP sequence = TCAGTCTGAGTACTAATCAAA Parent 2 SNP sequence = TCAGTCTGAG-ACTAATCAAA Recognized sequence for CAPs locus = GTAC
Probe for Locus	bah27d18
Locus	k03859
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = OSJNBb0061C13.7 [Oryza sativa (japonica cultivar-group)]
Type	PCR
PCR primers	Left 5' - CGACTCTTTCTGCCGGTTAG Right 5' - TTCTCTGTCAATGAGCGTGG
Sequence	AV945581
PCR size	357