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GrainGenes Marker Report: k04082

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Locus
k04082
Type
CAPS
Chromosome
7H
Map
Hv-HNxH602-7H136.4
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bah36f01
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = BstUI
SNP = A/G
SNP structure (UNICODE) = CAGTTCCTCTRGAACAGAGCT
Parent 1 SNP sequence = CAGTTCCTCTAGAACAGAGCT
Parent 2 SNP sequence = CAGTTCCTCTGGAACAGAGCT
Recognized sequence for CAPs locus = CGCG
SNP = T/G
SNP structure (UNICODE) = CGGGTGTAGTKGCACCAGCCG
Parent 1 SNP sequence = CGGGTGTAGTTGCACCAGCCG
Parent 2 SNP sequence = CGGGTGTAGTGGCACCAGCCG



Probe for Locus
bah36f01
Locus
k04082
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = NOD26-like membrane integral protein ZmNIP2-3 [Zea mays]
Type
PCR
PCR primers
Left 5' - CCAGCTGTAGCTCCGAAAAC
Right 5' - CCACCACCAAGGAAGACATC
Sequence
AV946827
PCR size
351