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GrainGenes Marker Report: k04865

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Locus
k04865
Type
CAPS
Chromosome
7H
Map
Hv-HNxH602-7H296.1
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
basd15d20
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = AfaI
SNP = G/A
SNP structure (UNICODE) = TCATGGAGTCRTACCTCATGA
Parent 1 SNP sequence = TCATGGAGTCGTACCTCATGA
Parent 2 SNP sequence = TCATGGAGTCATACCTCATGA
Recognized sequence for CAPs locus = GTAC



Probe for Locus
basd15d20
Locus
k04865
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = calcium-binding EF hand family protein [Arabidopsis thaliana] gb AAF80620.1 F2D10.25 [Arabidopsis thaliana]
Type
PCR
PCR primers
Left 5' - CAGAACACCTTGCTGCTCAA
Right 5' - CATGATCGCACTTGAGGTGA
Sequence
AV918987
PCR size
417