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GrainGenes Marker Report: k05030

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Locus
k05030
Type
CAPS
Chromosome
4H
Map
Hv-HNxH602-4H137
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
basd1d10
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = BstUI
SNP = G/C
SNP structure (UNICODE) = AGAGATTTCGSGACGTACCAG
Parent 1 SNP sequence = AGAGATTTCGGGACGTACCAG
Parent 2 SNP sequence = AGAGATTTCGCGACGTACCAG
Recognized sequence for CAPs locus = CGCG



Probe for Locus
basd1d10
Locus
k05030
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = alpha-tubulin 5 [Hordeum vulgare subsp. vulgare]
Type
PCR
PCR primers
Left 5' - CCAACACAGAGACAGGCTCA
Right 5' - AATCCAGATTCCCAGCACAG
Sequence
AV927257
PCR size
374