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GrainGenes Marker Report: k06728

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Locus	k06728
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 276.1
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaAK33K05
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = BcnI SNP = G/T SNP structure (UNICODE) = TGTTGCTCCCKGTCACAACAT Parent 1 SNP sequence = TGTTGCTCCCGGTCACAACAT Parent 2 SNP sequence = TGTTGCTCCCTGTCACAACAT Recognized sequence for CAPs locus = CCSGG
Probe for Locus	BaAK33K05
Locus	k06728
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = similar to transitin [Mus musculus]
Type	PCR
PCR primers	Left 5' - TGCACGTAAGCATCTTCAGC Right 5' - ACCTCCAGCTCCTCCTCTTC
Sequence	BJ458841
PCR size	399