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GrainGenes Marker Report: k07042

Locus
k07042
Type
CAPS
Chromosome
4H
Map
Hv-HNxH602-4H177.6
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
BaAL40L16
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = MboI
SNP = A/T
SNP structure (UNICODE) = TATACTCCGGWTCTTCATGCT
Parent 1 SNP sequence = TATACTCCGGATCTTCATGCT
Parent 2 SNP sequence = TATACTCCGGTTCTTCATGCT
Recognized sequence for CAPs locus = GATC



Probe for Locus
BaAL40L16
Locus
k07042
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = ribulose-phosphate 3-epimerase, chloroplast, putative / pentose-5-phosphate 3-epimerase, putative [Arabidopsis
Type
PCR
PCR primers
Left 5' - GAAAAACCCCAATCACTTGC
Right 5' - TGGCAACTTCCCATATCCTC
Sequence
BJ477227
PCR size
400