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GrainGenes Marker Report: k07221

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Locus	k07221
Type	CAPS
Chromosome	7H
Map	Hv-HNxH602-7H 212.9
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaAL19B06
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = TaqI SNP = G/C SNP structure (UNICODE) = ATATGTTCTCSACAGCCCGCA Parent 1 SNP sequence = ATATGTTCTCGACAGCCCGCA Parent 2 SNP sequence = ATATGTTCTCCACAGCCCGCA Recognized sequence for CAPs locus = TCGA
Probe for Locus	BaAL19B06
Locus	k07221
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = Eukaryotic initiation factor 4A-6 (eIF4A-6) (eIF-4A-6) emb CAA55740.1 unnamed protein product [Nicotiana tabacum]
Type	PCR
PCR primers	Left 5' - AGCAACGACAAACATTTCCA Right 5' - GATTTGATCAGCTGGGTGGT
Sequence	BJ475222
PCR size	355