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GrainGenes Marker Report: k07300

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Locus	k07300
Type	CAPS
Chromosome	3H
Map	Hv-HNxH602-3H 20.7
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaAL17J24
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = AvaII SNP = A/G SNP structure (UNICODE) = CGACGTACTGRTCCTGGCAGA Parent 1 SNP sequence = CGACGTACTGATCCTGGCAGA Parent 2 SNP sequence = CGACGTACTGGTCCTGGCAGA Recognized sequence for CAPs locus = GGWCC
Probe for Locus	BaAL17J24
Locus	k07300
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = Bowman-Birk type trypsin inhibitor pir TIBHB trypsin inhibitor (Bowman-Birk) - two-rowed barley
Type	PCR
PCR primers	Left 5' - CTTACGCAGCAATCTTGTCG Right 5' - TTACAACCGAGAAACCGACC
Sequence	AJ486153
PCR size	429