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GrainGenes Marker Report: k08607

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Locus
k08607
Type
CAPS
Chromosome
5H
Map
Hv-HNxH602-5H32.4
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
BaH47G19
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = TaqI
SNP = G/A
SNP structure (UNICODE) = TGGCGGCCTCRACCATGTTGG
Parent 1 SNP sequence = TGGCGGCCTCGACCATGTTGG
Parent 2 SNP sequence = TGGCGGCCTCAACCATGTTGG
Recognized sequence for CAPs locus = TCGA



Probe for Locus
BaH47G19
Locus
k08607
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = methionine synthase protein [Sorghum bicolor]
Type
PCR
PCR primers
Left 5' - ACATCCTTGCTTGCTTGCTT
Right 5' - CACCCAGTGGCTCTGGTACT
Sequence
BM372629
PCR size
350