Query (optional)   in Class  

GrainGenes Marker Report: k08617

[Submit comment/correction]

Locus
k08617
Type
CAPS
Chromosome
7H
Map
Hv-HNxH602-7H186.4
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
BaH27K19
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
[ Hide all but 1 of 6 ]
Restriction enzyme used for CAPS analysis = BsmAI
SNP = G/A
SNP structure (UNICODE) = TCTAAGAAGGRGACTCGTGTC
Parent 1 SNP sequence = TCTAAGAAGGGGACTCGTGTC
Parent 2 SNP sequence = TCTAAGAAGGAGACTCGTGTC
Recognized sequence for CAPs locus = NNNNNGAGAC



Probe for Locus
BaH27K19
Locus
k08617
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = thiamine biosynthetic enzyme [Oryza sativa (japonica cultivar-group)]
Type
PCR
PCR primers
Left 5' - AAGCATTCCCGAAGCAGTAA
Right 5' - ACCCAGATCAAAATGTTCGC
Sequence
BJ482468
PCR size
350