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GrainGenes Marker Report: k09350

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Locus	k09350
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 212.6
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaH57L13
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = AfaI SNP = A/G SNP structure (UNICODE) = TCGGACGGTARTACTTGTTGG Parent 1 SNP sequence = TCGGACGGTAATACTTGTTGG Parent 2 SNP sequence = TCGGACGGTAGTACTTGTTGG Recognized sequence for CAPs locus = GTAC
Probe for Locus	BaH57L13
Locus	k09350
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = putative N5'-nucleotidase [Oryza sativa (japonica cultivar-group)] gb AAP68896.1 putative N5'-nucleotidase [Oryza sativa (japonica cultivar
Type	PCR
PCR primers	Left 5' - GAACCAGGCCTTCATCAGAG Right 5' - TCCACCATCATCGTCGAGTA
Sequence	BJ552636
PCR size	424