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GrainGenes Marker Report: k09976

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Locus
k09976
Type
CAPS
Chromosome
2H
Map
Hv-HNxH602-2H31.1
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bast42A0602
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = HapII
SNP = C/T
SNP structure (UNICODE) = GCACCACCCGYCGGCCGTCGA
Parent 1 SNP sequence = GCACCACCCGCCGGCCGTCGA
Parent 2 SNP sequence = GCACCACCCGTCGGCCGTCGA
Recognized sequence for CAPs locus = CCGG
SNP = T/G
SNP structure (UNICODE) = GGCAGCAGCCKGTGCGGCACC
Parent 1 SNP sequence = GGCAGCAGCCTGTGCGGCACC
Parent 2 SNP sequence = GGCAGCAGCCGGTGCGGCACC



Probe for Locus
bast42A0602
Locus
k09976
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = oxo-phytodienoic acid reductase [Oryza sativa (japonica cultivar-group)]
Type
PCR
PCR primers
Left 5' - GGAAGACACGGAAACTGGAA
Right 5' - GATGACTGCTCTGCAGCTTG
Sequence
AL502102
PCR size
317