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GrainGenes Reference Report: PJB-36-629

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Reference	PJB-36-629
Title	Plant regeneration by somatic embryogenesis from callus of mature seed explants of bread wheat ( Triticum aestivum L.)
Journal	Pakistan Journal of Botany
Year	2004
Volume	36
Pages	629-634
Author	Malik SI Hamid R Yasmin T Minhas NM
Abstract	Whole-plant regeneration by somatic embryogenesis was studied in wheat cultivars Inqilab-91 and Pavon-76. Embryogenic callus was obtained by culturing the seeds of both cultivars on gel-solidified Linsmaier and Skoog's medium with 3.5 mg 2,4-D/litre. The callus cultures were maintained by subsequent subcultures onto fresh media at 4- to 5-week intervals. The embryogenic calluses cleared of any surrounding non-embryogenic callus were grown on gel-solidified basic Murashige and Skoog's medium with 3% (w/v) sucrose after at least 2 subcultures. Seven combinations of BAP (6-benzylaminopurine [benzyladenine]) and IAA (0 + 0.1, 0.5 + 0.1, 2.5 + 0.1, 5.0 + 0.1, 0.5 + 0, 2.5 + 0 and 5.0 + 0 mg/litre) and one control were assayed. The highest frequency of green spot formation and plant regeneration (84% in Inqilab-91 and 52% in Pavon-76) were obtained with 0.5 + 0.1 mg BAP + IAA/litre. Higher concentrations of BAP in the media was toxic and fatal for calluses in some cases. Significant variation in callus initiation and in vitro regeneration was observed between the cultivars
Keyword	[ Hide all but 1 of 22 ] 2,4-d agriculture application rates benzyladenine bread callus control cultivar culture media embryogenesis explants iaa in vitro culture in vitro regeneration plant breeding plant growth regulators regeneration seed somatic embryogenesis sucrose tissue culture varietal reactions

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