Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PLA-207-590

[Submit comment/correction]

Reference	PLA-207-590
Title	Genes controlling hydroxylations of phytosiderophores are located on different chromosomes in barley (Hordeum vulgare L.).
Journal	Planta
Year	1999
Volume	207
Pages	590-596
Author	Ma JF [ Show all 7 ]
Abstract	Phytosiderophores, mugineic acids, have been demonstrated to be involved in Fe acquisition in gramineous plants. In this study, chromosomal arm locations of genes encoding for biosynthesis of various phytosiderophores were identified in a cultivar of barley (Hordeum vulgare L. cv. Betzes). Using wheat (Triticum aestivum L. cv. Chinese Spring)-barley (cv. Betzes) ditelosomic addition lines for 4HS and 4HL, a gene for hydroxylation of 2'-deoxymugineic acid to mugineic acid was localized to the long arm of barley chromosome 4H. To locate the gene for hydroxylation of mugineic acid to 3-epihydroxymugineic acid, hybrids between the 4H addition line and other wheat-barley addition lines were studied. Only a hybrid between 4H and 7H addition lines produced 3-epihydroxymugineic acid. The gene was further localized to the long arm of chromosome 7H by feeding mugineic acid to ditelosomic addition lines for 7HS and 7HL. A new phytosiderophore was discovered in both 7H and 7HL addition lines, which was identified to be 3-epihydroxy-2'-deoxymugineic acid by detailed nuclear magnetic resonance studies. These results revealed that in barley there are two pathways from 2'-deoxymugineic acid to 3-epihydroxymugineic acid: 2'-deoxymugineic acid leads to mugineic acid leads to 3-epihydroxymugineic acid and 2'-deoxymugineic acid leads to 3-epihydroxy-2'-deoxymugineic acid leads to 3-epihydroxymugineic acid. Barley genes encoding for the hydroxylations of phytosiderophores are located in different chromosomes and each gene hydroxylates different C-positions: the long arm of chromosome 4H carries the gene for hydroxylating the C-2' position and the long arm of chromosome 7H carries the gene for hydroxylating the C-3 position of the azetidine ring.
External Databases	Agricola: 2008-23560
Keyword	[ Hide all but 1 of 10 ] addition lines biosynthesis chemical reactions chromosome maps gene location hordeum vulgare iron nutrient uptake siderophores triticum aestivum

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.