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GrainGenes Reference Report: PLC-57-121

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Reference	PLC-57-121
Title	Cold pretreatment to enhance green plant regeneration from rye anther culture
Journal	Plant Cell
Year	2000
Volume	57
Pages	121-127
Author	Immonen S Anttila H
Abstract	Summary: Cold pretreatment of detached tillers of rye, Secaley cereale, was tested under two light regimes. The ryes included two spring and two winter cultivars. Significant increases in green plant regeneration were recorded in each experiment when cold pretreatments of two to four weeks were applied. Dim light during the stress period improved green plant regeneration for two of the four cultivars tested. The highest regeneration rate, 30.6 green plants per 100 plated anthers, was reached following three weeks at +4 degrees C under dim light, for spring rye Jo02. Starvation stress applied to plated anthers in mannitol medium suppressed anther response
Keyword	[ Hide all but 1 of 22 ] 2,4 d anther culture benzyladenine chilling cold culture media developmental stages dosage effects embryonic development gametes genetic variation kinetin light mannitol naa plant morphology regenerative ability secale cereale stress temperature tillers winter

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