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GrainGenes Reference Report: PLC-61-153

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Reference	PLC-61-153
Title	High frequency androgenesis in indica x Basmati rice hybrids using liquid culture media
Journal	Plant Cell
Year	2000
Volume	61
Pages	153-159
Author	Bishnoi US Jain RK Gupta KR Chowdhury VK Chowdhury JB
Abstract	Summary: An improved procedure has been developed for high frequency androgenesis in indica X Basmati rice hybrids using a liquid culture medium. Anthers from fourteen genotypes comprising of indica X Basmati rice F1 hybrids, F2 plants and the parental rice cultivars, were floated in liquid RZM, N6M, and Heh5M media. Anther culture frequencies (percentage of anthers forming calluses) in most of the genotypes were significantly higher in RZM medium (16-75%) compared to those obtained in N6M (7-29%) and Heh5M (7-41%) media. Agarose (1.0% w/v)-solidified MSR1 medium containing 3.0% (w/v) maltose, 1 mg l-1 kinetin, 1 mg l-1 6-benzyladenine (BA) and 0.5 mg l-1 alpha-naphthalene acetic acid (NAA) induced green shoot regeneration at high frequencies compared to the medium (MSR2) lacking BA. In all the genotypes, microspore calluses initiated in RZM medium regenerated green shoots with over tenfold higher frequencies compared to the calluses initiated in other two media. High plant regeneration frequencies (up to 270 green plants/1000 anthers) were obtained from microspore-derived calluses of some of the F1 hybrids (Gobind X Basmati 370, Gobind X Taraori Basmati) and F2 plants (Gobind X Basmati 370, Gobind X Taraori Basmati, HKR86-3 X Taraori Basmati) as compared to their actual parents. Cytological analysis of the root tips of the progeny seedlings of the microspore-derived plants revealed haploids at a frequency of about 50%; 22% of the microspore-derived plants had > 5% spikelet fertility and were diploid. Use of RZM liquid and MSR1 media, respectively for anther culture and plant regeneration resulted in several fold increase in the recovery of green plants from recalcitrant indica X Basmati rice F1 hybrids/F2 plants which were comparable to those reported for japonica rice varieties/hybrids leading to the improved feasibility of using doubled haploids in genetic, breeding and mapping research with indica rice
Keyword	[ Hide all but 1 of 38 ] 2,4 d androgenesis anther culture basmati rice benzyladenine breeding culture culture media dosage effects doubled haploid fertility frequencies genetic variation genotype green haploid hybrid indica indica rice japonica kinetin liquid culture maltose mapping media micropropagation naa oryza sativa plant regeneration progeny regeneration regenerative ability rice root root tips seedlings shoot shoot regeneration

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