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GrainGenes Reference Report: PLC-65-9

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Reference	PLC-65-9
Title	Cell culture induced introgression of Thinopyrum intermedium chromatin into common wheat
Journal	Plant Cell
Year	2000
Volume	65
Pages	9-13
Author	Liu B Luan Y Han F Ji W He M
Abstract	Summary: To introduce useful genes from the wild species Thinopyrum intermedium into cultivated wheat, a wheat-Th. intermedium disomic addition line (TAI27) was used as source material for tissue culture. TAI27 contains, beside the 42 wheat chromosomes, a pair of smaller chromosomes that is cytologically discernible. Based on restriction fragment length polymorphism (RFLP) analysis, this chromosome pair was determined to be a recombinant one, comprising segments with homoeology to at least two chromosome groups of wheat, i.e., group 2 and 7. Sixty-eight green plants were regenerated from six month-old embryogenic calli initiated from immature embryos of TAI27. Four of the plants were found to have only 42 cytologically normal-looking chromosomes. Southern blot analysis using a Th. intermedium-enriched repetitive probe showed that one of the plants had hybridization fragments specific to Th. intermedium, indicating introgression of chromatin during the cell culture process
Keyword	[ Hide all but 1 of 34 ] addition callus cell cell culture chromatin chromosome chromosome number common wheat culture elymus hispidus embryo fragment length polymorphism fragments gene gene transfer homoeology hybridization immature embryo intermedium introgression length polymorphism polymorphism regenerative ability restriction restriction fragment length rflp thinopyrum thinopyrum intermedium tissue tissue culture triticum aestivum wheat chromosomes wild wild species

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