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GrainGenes Reference Report: PLC-66-79

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Reference	PLC-66-79
Title	Transformation of oat and inheritance of bar gene expression
Journal	Plant Cell
Year	2000
Volume	66
Pages	79-88
Author	Kuai B [ Show all 6 ]
Abstract	Summary: Fertile transgenic plants of oat (Avena sativa L. var. Melys) were produced following microprojectile bombardment of primary embryogenic calli from immature embryos with two plasmids containing the bar gene or the beta-glucuronidase (uidA) gene, after selection with glufosinate ammonium. Eleven plants were regenerated from phosphinothricin resistant callus, with three of the eleven plants containing either intact or rearranged copies. No plants co-transformed with the non-selected uidA gene were detected. Stable transmission and expression of the bar gene in the T1 inbred progenies occurred in a Mendelian manner in one line, which contained an intact bar gene, and in all six T2 lines tested from this transformant
Keyword	[ Hide all but 1 of 39 ] acyltransferases ammonia ammonium avena avena sativa bar bar gene beta-glucuronidase bombardment callus embryo enzyme activity fertile fertile transgenic plants gene gene expression gene transfer genetic transformation glufosinate herbicide resistance immature embryo in vitro culture inbred lines inheritance microprojectile bombardment oat phosphinothricin plant embryos plasmid progeny regenerative ability resistant selection transformation transgenic transgenic plant transmission uida uida gene

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