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GrainGenes Reference Report: PLC-73-57

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Reference	PLC-73-57
Title	Production of multiple shoots from thidiazuron-treated mature embryos and leaf-base/apical meristems of barley (Hordeum vulgare)
Journal	Plant Cell
Year	2000
Volume	73
Pages	57-64
Author	Ganeshan S [ Show all 6 ]
Abstract	Summary: The in vitro plant regeneration frequencies for immature scutella, leaf-bases/apical meristems (LB/AM) and mature embryos of four commercially important barley genotypes were compared. Production of shoots from mature embryos or calluses of LB/AM incubated on media containing 1.0 or 2.0 mg l-1 6-benzylaminopurine (BA) were comparable to regeneration frequencies obtained for scutella-derived calluses of the same genotypes. Incubation of excised mature embryos and LB/AM on media containing the plant growth regulator, thidiazuron (TDZ), resulted in an increased shoot production. However, TDZ treatment did not stimulate plant regeneration from calluses derived from scutella or LB/AM. Shoots formed from TDZ-treated mature embryos and LB/AM were induced without a callus interphase and the in vitro culture system gave a three- to eight-fold higher regeneration frequency than recorded for scutella-derived calluses on BA medium. The simplicity and rapid development of shoots using the mature embryo system could potentially be used for the regeneration and genetic transformation of barley over alternative regeneration systems
Keyword	[ Hide all but 1 of 31 ] 2,4 d apical meristem barley benzyladenine callus culture media dose response embryo embryo (plant)k explants frequencies genetic transformation genetic variation genetic-transformation genotype growth hordeum hordeum vulgare in vitro culture in vitro regeneration incubation media meristem methodology micropropagation plant growth plant growth substances regeneration regulator scutellum transformation

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