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GrainGenes Reference Report: PLT-229-323

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Reference	PLT-229-323
Title	Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization.
Journal	Planta
Year	2009
Volume	229
Pages	323-330
Author	[ Hide all but 1 of 6 ] Cui H Yu Z Deng J Gao X Sun Y Xia G
Abstract	Regenerates were obtained following somatic hybridization between tall wheatgrass (Agropyron elongatum) and bread wheat (Triticum aestivum cv. Jinan177) protoplasts. Two lines (CU and XI) were self-fertile in the first (R(0)) and subsequent (R(1) and R(2)) generations. The phenotype of each R(1) population was uniform. All CU progeny were phenotypically similar to the tall wheatgrass parent, while XI progeny had thinner, smoother and softer leaves. Cytological analysis showed that more wheat chromatin was present in the hybrid callus than in the R(1) and R(2) plants, and that some intercalary translocations of wheat chromosome segments were retained in the R(2) generation. AFLP profiling confirmed the presence of wheat DNA in the introgression lines. Analysis of the high molecular weight glutenin subunit content of derived seed identified three novel subunits, not present in either the wheat or the tall wheatgrass parent. Microsatellite-based profiling of the chloroplast genome of the introgression lines suggested that only chloroplast sequences from the tall wheatgrass parent were present. The specifically inherited phenomena and possible application of these hybrids are discussed.
External Databases	http://dx.doi.org/10.1007/s00425-008-0832-z

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