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GrainGenes Reference Report: PMB-17-509

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Reference	PMB-17-509
Title	A copy of exon 3-intron 3 from the barley aleurain gene is present on chromosome 2.
Journal	Plant Molecular Biology
Year	1991
Volume	17
Pages	509-512
Author	Li XY Rogers SW Rogers JC
Abstract	A genomic clone from Hordeum vulgare L. cv. Himalaya contains 700 bp of DNA that is homologous with a high degree of nucleotide sequence similarity to exon 3-intron 3 from the gene for the thiol protease, aleurain. Genomic Southern blot mapping data indicate that this clone in phage .lambda. had not undergone rearrangement, and no other sequences homologous to aleurain are present on it. Although exon 3 in aleurain encodes the polypeptide region cleaved during proteolytic processing of the proenzyme to its mature form, we do not known if the copy is expressed in some other protein. We have mapped the aleurain gene to chromosome 1, and this copy of exon 3- intron 3 to chromosome 2.
Gene	Ale1 (Hordeum)
Probe	BamHI/SpeI
Keyword	[ Hide all but 1 of 10 ] cultivar Himalaya DNA embl genbank Hordeum vulgare nucleotide sequence polypeptide protease proteolytic processing southern blot mapping data

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