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GrainGenes Reference Report: PMB-27-435

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Reference	PMB-27-435
Title	An extrachromosomal fragment of telomeric DNA in wheat
Journal	Plant Molecular Biology
Year	1995
Volume	27
Pages	435-439
Author	Bucholc M Buchowicz J
Abstract	A procedure developed originally for selective extraction of viral (extrachromosomal) DNA from virus-infected mammalian cells was applied to cell nuclei isolated from uninfected wheat embryos The resulting nuclear extrachromosomal DNA (exDNA) was enriched for telomere-type sequences by isopycnic centrifugation and inserted into the Sma I site of pUC119. A cloned DNA fragment (241 bp) was found to consist primarily of tandemly repeated heptamere units of the same sequence (5'-CCCTAAA3') that is known to predominate in telomeric DNA of Arabidopsis thaliana. Hybridization experiments indicate that extrachromosomal telomeric repeats are abundant in resting embryos and disappear rapidly during germination.
Keyword	[ Hide all but 1 of 11 ] cloning dna hybridization extrachromosomal-dna extrachromosomal-telomeric-repeats extraction plant embryos repetitive dna seed germination separation telomere-type-sequences triticum aestivum

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