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GrainGenes Reference Report: PMB-41-777

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Reference	PMB-41-777
Title	Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination
Journal	Plant Molecular Biology
Year	1999
Volume	41
Pages	777-783
Author	Nuutila AM Ritala A Skadsen RW Mannonen L Kauppinen V
Abstract	Summary: The malting quality of two barley cultivars, Kymppi and Golden Promise, was modified to better meet the requirements of the brewing process. The egl1 gene, coding or fungal thermotolerant endo-1,4-beta-glucanase (EGI, cellulase), was transferred to the cultivars using particle bombardment, and transgenic plants were regenerated on bialaphos selection. Integration of the egl1 gene was confirmed by Southern blot hybridization. The transgenic seeds were screened for the expression of the heterologous EGI. Under the high-pI alpha-amylase promoter, the egl1 gene was expressed during germination. The heterologous enzyme was thermotolerant at 65 degrees C for 2 h, thus being suitable for mashing conditions. The amount of heterologous EGI produced by the seeds (ca. 0.025% of soluble seed protein), has been shown to be sufficient to reduce wort viscosity by decreasing the soluble beta-glucan content. A decrease in the soluble beta-glucan content in the wort improves the filtration rate of beer
Keyword	[ Hide all but 1 of 38 ] alpha amylase barley barley cultivars beer beta-glucan biolistics blot hybridization bombardment brewing cellulase enzyme fungal gene gene expression gene transfer genetic transformation germination heat tolerance hordeum vulgare hybridization integration malting malting barley malting quality particle bombardment promoter quality recombinant dna regenerative ability seed seed germination selection transgenic transgenic barley transgenic plant trichoderma longibrachiatum viscosity wort

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