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GrainGenes Reference Report: PMB-45-691

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Reference	PMB-45-691
Title	Survey of gene expression in winter rye during changes in growth temperature, irradiance or excitation pressure
Journal	Plant Molecular Biology
Year	2001
Volume	45
Pages	691-703
Author	Ndong C Danyluk J Huner NPA Sarhan F
Abstract	Summary: Previous comparisons of winter rye plants (Secale cereale L. cv. Musketeer) grown in a combination of specific temperature (degrees C)/irradiance (micromol m-2 s-1) regimes (20/50; 20/250; 20/800; 5/50; 5/250) revealed (1) that photosynthetic acclimation to low temperature mimics photosynthetic acclimation to high light because both conditions result in comparable reduction states of photosystem II (PSII), that is, comparable PSII excitation pressure; (2) that the relative redox state of PSII also appears to regulate a specific cold acclimation gene, Wcsl9. In order to identify additional genes regulated differentially by either low temperature, irradiance or excitation pressure, we initiated a detailed analysis of gene expression. We identified and characterized 42 differentially expressed genes from wheat and rye. Based on their patterns of regulation under the five growth conditions employed, 37 of the cDNAs could be classified into four groups: genes regulated by PSII excitation pressure, low temperature, growth irradiance and interaction between growth temperature and irradiance. Partial sequence analyses revealed that several of these genes encode known chloroplastic proteins such as ELIPs, transketolase, carbonic anhydrase and Mg-chelatase. However, five of the genes could not be classified unambiguously into any one of these four categories. The implications of these results and the limitations of the experimental design are discussed in terms of larger-scale genomic studies designed to understand the interactions of multiple abiotic stresses to which a plant may be exposed when examining regulation of gene expression
Keyword	[ Hide all but 1 of 46 ] abiotic stress acclimation blue-light calvin cycle cdna cdnas chlorella-vulgaris cold cold acclimation design differentially expressed genes excitation pressure experimental design freezing tolerance gene gene expression growth growth conditions harvesting complex-ii hordeum vulgare l. interaction irradiance light limitation low temperature messenger-rna abundance multiple pattern photosynthetic acclimation photosystem ii plant science plasma membrane protein reduction regulation regulation of gene expression rye secale secale cereale sequence sequence analyses state stress temperature winter winter rye

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