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GrainGenes Reference Report: PNA-93-5624

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Reference	PNA-93-5624
Title	A novel iron-regulated metal transporter from plants identified by functional expression in yeast
Journal	Proceedings of the National Academy of Sciences, USA
Year	1996
Volume	93
Pages	5624-5628
Author	Eide D Broderius M Fett J Guerinot M
Abstract	Iron is an essential nutrient for virtually all organisms The IRT1 (iron-regulated transporter) gene of the plant Arabidopsis thaliana, encoding a probable Fe(II) transporter, was cloned by functional expression in a yeast strain defective for iron uptake Yeast expressing IRT1 possess a novel Fe(II) uptake activity that is strongly inhibited by Cd IRT1 is predicted to be an integral membrane protein with a metal-binding domain Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis Related sequences were also found in the genomes of rice, yeast, nematodes, and humans In Arabidopsis, IRT1 is expressed in roots, is induced by iron deficiency, and has altered regulation in plant lines bearing mutations that affect the iron uptake system. These results provide the first molecular insight into iron transport by plants.
Keyword	[ Hide all but 1 of 27 ] amino acid sequence arabidopsis thaliana binding protein cloning complementary dna complementation ferrous-ions gene expression ion-transport ion-uptake.inhibition iron-transporter irt1 gene membrane protein messenger rna metal-ions mineral-uptake molecular sequence data multigene-families mutation nucleotide sequence nutrient-availability plant proteins plasma-membranes root saccharomyces cerevisiae shoots structural genes

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