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GrainGenes Reference Report: PNA-96-11117

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Reference	PNA-96-11117
Title	Single-copy transgenic wheat generated through the resolution of complex integration patterns
Journal	Proceedings of the National Academy of Sciences, USA
Year	1999
Volume	96
Pages	11117-11121
Author	Srivastava V Anderson OD Ow DW
Abstract	Summary: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes
Keyword	[ Hide all but 1 of 32 ] alleles bilanafos biolistics chimera dna gene gene dosage gene expression gene transfer genetic transformation genetic vectors genome herbicide resistance integration inversion (genetics) loci locus nucleases plasmid vectors recombination recombination,genetic reporter gene segregation site-specific recombination transformation transgene transgenes transgenic transgenic plant transgenic wheat triticum aestivum wheat transformation

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