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GrainGenes Reference Report: PNA-96-15348

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Reference	PNA-96-15348
Title	A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription
Journal	Proceedings of the National Academy of Sciences, USA
Year	1999
Volume	96
Pages	15348-15353
Author	Hobo T Kowyama Y Hattori T
Abstract	Summary: The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcription from a chimeric promoter with GAL4-binding sites was ABA-inducible if cells expressed a GAL4 DNA-binding domain::TRAB1 fusion protein. Results indicate that TRAB1 is a true trans-acting factor involved in ABA-regulated transcription and reveal a molecular mechanism for the VP1-dependent, ABA-inducible transcription that controls maturation and dormancy in plant embryos
Keyword	[ Hide all but 1 of 16 ] abscisic acid beta glucuronidase binding sites complementary dna gene expression genetic regulation messenger rna oryza sativa plant embryos protoplast recombinant dna regulatory sequences reporter gene stress transcription transgenic plant

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