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GrainGenes Reference Report: PPS-122-57

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Reference	PPS-122-57
Title	Multiple mode regulation of a cysteine proteinase gene expression in rice
Journal	Plant Physiology
Year	2000
Volume	122
Pages	57-66
Author	Ho SL Tong WF Yu SM
Abstract	Summary: In many plants, cysteine proteinases play essential roles in a variety of developmental and physiological processes. In rice (Oryza sativa), REP-1 is a primary cysteine proteinase responsible for the digestion of seed storage proteins to provide nutrients to support the growth of young seedlings. In the present study, the gene encoding REP-1 was isolated, characterized, and designated as OsEP3A. An OsEP3A-specific DNA probe was used to study the effect of various factors on the expression of OsEP3A in germinating seeds and vegetative tissues of rice. The expression of OsEP3A is hormonally regulated in germinating seeds, spatially and temporally regulated in vegetative tissues, and nitrogen-regulated in suspension-cultured cells. The OsEP3A promoter was linked to the coding sequence of the reporter gene, gusA, which encodes beta-glucuronidase (GUS), and the chimeric gene was introduced into the rice genome. The OsEP3A promoter is sufficient to confer nitrogen regulation of GUS expression in suspension-cultured cells. Histochemical studies also indicate that the OsEP3A promoter is sufficient to confer the hormonal regulation of GUS expression in germinating seeds. These studies demonstrate that in rice the REP-1 protease encoded by OsEP3A may play a role in various physiological responses and processes, and that multiple mechanisms regulate the expression of OsEP3A
Keyword	[ Hide all but 1 of 42 ] amino acid sequence beta glucuronidase beta-glucuronidase cell cell suspensions chimeric gene cysteine cysteine proteinase digestion dna gene gene expression genetic regulation genome growth gus gus expression histoenzymology mechanism messenger rna nitrogen nutrients oryza sativa plant tissues promoter protease proteinase regulation reporter gene rice rice genome seed seed germination seed storage protein sequence storage storage protein suspension-cultured cells temporal variation tissue transgenic plant vegetative tissues

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