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GrainGenes Reference Report: PPS-122-255

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Reference	PPS-122-255
Title	Wheat granule-bound starch synthase I and II are encoded by separate genes that are expressed in different tissues
Journal	Plant Physiology
Year	2000
Volume	122
Pages	255-263
Author	Vrinten PL Nakamura T
Abstract	Summary: Studies of waxy mutations in wheat and other cereals have shown that null mutations in genes encoding granule-bound starch synthase I (GBSSI) result in amylose-free starch in endosperm and pollen grains, whereas starch in other tissues may contain amylose. We have isolated a cDNA from waxy wheat that encodes GDSSII, which is thought to be responsible for the elongation of amylose chains in non-storage tissues. The deduced amino acid sequences of wheat GBSSI and GBSSII were almost 66% identical, while those of wheat GBSSII and potato GBSSI were 72% identical. GBSSII was expressed in leaf, culm, and pericarp tissue, but transcripts were not detected in endosperm tissue. In contrast, GBSSI expression was high in endosperm tissue. The expression of GBSSII mRNA in pericarp tissue was similar at the midpoints of the day and night periods. The GBSSII genes were mapped to chromosomes 2AL, 2B, and 2D, whereas GBSSI genes are located on group 7 chromosomes. Gelblot analysis indicated that genes related to GBSSII also occur in barley, rice, and maize. The possible role of GBSSII in starch synthesis is discussed
Keyword	[ Hide all but 1 of 35 ] amino acid amino acid sequence amylose barley cdna chromosome maps complementary dna elongation endosperm gene gene expression gene location genetic mapping grains granule-bound starch synthase group-7 hexosyltransferases maize mrna mutation pericarp plant tissues pollen potato rice sequence starch starch synthase stem synthase synthase-i synthesis tissue transcript triticum aestivum

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