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GrainGenes Reference Report: PPS-125-1228

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Reference	PPS-125-1228
Title	Mapping maize sequences to chromosomes using oat-maize chromosome addition materials
Journal	Plant Physiology
Year	2001
Volume	125
Pages	1228-1235
Author	Okagaki RJ [ Show all 6 ]
Abstract	Summary: Oat- (Avena sativa) maize (Zea mays) chromosome additions are produced by crossing maize and oat. During early embryo development maize chromosomes are preferentially eliminated, and oat plants are often recovered that retain a single maize chromosome. Each of the 10 maize chromosomes recently has been isolated as a separate oat-maize addition. We describe here the mapping of 400 maize sequences to chromosomes using polymerase chain reaction and DNA from the oat-maize addition material. Fifty of the sequences were from cloned markers that had been previously mapped by linkage analysis, and our results were consistent with those obtained using Southern-blot analysis. Previously unmapped expressed sequence tags and sequence tagged sites (350) were mapped to chromosomes. Maize gene sequences and expression data are rapidly being accumulated. Coupling this information with positional information from high throughput mapping programs provides plant biologists powerful tools for identifying candidate genes of interest
External Databases	Pubmed: 11244104
Keyword	[ Hide all but 1 of 36 ] avena avena sativa candidate gene chain chromosome chromosome addition crossing development dna embryo expressed sequence tags fragment length polymorphism gene gene sequence genome linkage linkage analysis linkage maps maize maize gene mapping marker mays oat polymerase polymerase chain reaction program programs rice sequence sequence tagged site sequence tags single tool zea zea mays

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