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GrainGenes Reference Report: PPS-135-1162

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Reference	PPS-135-1162
Title	Ice-cap. A high-throughput method for capturing plant tissue samples for genotype analysis
Journal	Plant Physiology
Year	2004
Volume	135
Pages	1162-1169
Author	Krysan P
Abstract	High-throughput genotype screening is rapidly becoming a standard research tool in the post-genomic era. A major bottleneck currently exists, however, that limits the utility of this approach in the plant sciences. The rate-limiting step in current high-throughput pipelines is that tissue samples from living plants must be collected manually, one plant at a time. I describe a novel method for harvesting tissue samples from living seedlings that eliminates this bottleneck. The method has been named Ice-Cap to reflect the fact that ice is used to capture the tissue samples. The planting of seeds, growth of seedlings, and harvesting of tissue are all performed in a 96-well format. I demonstrate the utility of this system by using tissue harvested by Ice-Cap to genotype a population of Arabidopsis thaliana seedlings that is segregating a previously characterized mutation. Because the harvesting of tissue is performed in a nondestructive manner, plants with the desired genotype can be transferred to soil and grown to maturity. I also show that Ice-Cap can be used to analyse genomic DNA from rice (Oryza sativa) seedlings. It is expected that this method will be applicable to high-throughput screening with many different plant species, making it a useful technology for performing marker-assisted selection
External Databases	Pubmed: 15266050
Keyword	[ Hide all but 1 of 15 ] arabidopsis assisted selection dna extraction growth mads-box genes marker mutation oryza pcr population rice seed seedling selection

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