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GrainGenes Reference Report: PRT-14-393

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Reference	PRT-14-393
Title	Efficient callus formation and plant regeneration from leaves of oats (Avena sativa L.)
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	393-397
Author	Chen H Xu G Loschke D Tomaska L Rolfe B
Abstract	An efficient plant regeneration system from leaf:derived callus in 6 genotypes of Avena sativa L has been established Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings Plants were regenerated from the regenerable callus and grown to maturity The frequency of regenerable callus formation and plant regeneration was correlated with the position, developmental stage and genotype of the explant The regeneration capacity of the first one mm of the leaf basal region from three day old seedlings was comparable to that of immature embryos Furthermore, the leaf regenerable calli were subcultured for 8 months without loss of their regeneration capabilities.
Keyword	[ Hide all but 1 of 9 ] avena sativa callus culture-media genetic variation genotype leaves plant embryos regenerative-ability tissue culture

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