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GrainGenes Reference Report: PRT-19-1000

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Reference	PRT-19-1000
Title	Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants
Journal	Plant Cell Reports
Year	2000
Volume	19
Pages	1000-1005
Author	Nobre J Davey MR Lazzeri PA Cannell ME
Abstract	Summary: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker uidA gene, and pCaIneo, which contains the selectable marker neomycin phosphotransferase gene. Protoplast-derived calluses were selected on medium containing the antibiotic G418 (25 and 15 mg.l-1) and macroscopic antibiotic resistant colonies were recovered. Fertile plants were regenerated from a callus line and molecular analysis confirmed transgene integration
Keyword	[ Hide all but 1 of 26 ] barley callus direct gene-transfer dna fertile gene hordeum vulgare integration maize malting marker media molecular analysis plasmids promoter protoplast regeneration resistant rice plants scutellum selectable marker stable transformation transformation transgene transgenic transgenic plant

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