Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PYC-37-113

[Submit comment/correction]

Reference	PYC-37-113
Title	The dynamic conformation of plant cytoplasmic 5S rRNAs
Journal	Phytochemistry
Year	1994
Volume	37
Pages	113-117
Author	Barciszewska M Erdmann V Barciszewski J
Abstract	Recently we have proposed a new three-dimensional model of plant 5S rRNAs structure To verify this proposal we present here new data on RNase T1 digestion and hydroxyl radical hydrolysis of lupin and wheat germ 5S rRNAs at various buffer and temperature conditions Interestingly, the guanosine residues 85-87 in the loop D of these RNAs, are resistant to RNase T1 at native but not at denaturating conditions On the other hand, the reaction of 5S rRNA with the hydroxyl radicals showed different reactivity of many nucleotides in various parts of the molecule and suggest conformational changes, which occur mostly in the loops The experimental data clearly support involvement of the nucleotides occupying conserved positions in the loops in the tertiary interactions in plant 5S rRNA structure
Keyword	[ Hide all but 1 of 11 ] chemical composition cytoplasm enzyme activity free-radicals hydrolysis molecular-conformation molecular-structure nucleotide plant physiology ribonucleases ribosomal-rna

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.